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Image Search Results
Journal: Human reproduction (Oxford, England)
Article Title: A novel selective progesterone receptor modulator asoprisnil (J867) down-regulates the expression of EGF, IGF-I, TGFbeta3 and their receptors in cultured uterine leiomyoma cells.
doi: 10.1093/humrep/del035
Figure Lengend Snippet: Figure 1. Effects of asoprisnil on the expression of immunoreactive epidermal growth factor (EGF), insulin-like growth factor-I (IGF-I) and transforming growth factor β (TGFβ3) protein in leiomyoma and normal myometrial cells cultured for 48 or 72 h, as assessed by immunocyto- chemistry. The expression of immunoreactive EGF, IGF-I and TGFβ3 protein in leiomyoma and normal myometrial cells cultured for 48 h (upper panel) and for 72 h (lower panel). EGF in leiomyoma cells cultured without any treatment (a, A), EGF in leiomyoma cells cultured in the presence of 10–7 M asoprisnil (d, D), IGF-I in leiomyoma cells cultured without any treatment (b, B), IGF-I in leiomyoma cells cultured in the presence of 10–7 M asoprisnil (e, E), TGFβ3 in leiomyoma cells cultured without any treatment (c, C) and TGFβ3 in leiomyoma cells cultured in the presence of 10–7 M asoprisnil (f, F). EGF in normal myometrial cells cultured without any treatment (g, G), EGF in normal myometrial cells cultured in the presence of 10–7 M asoprisnil (j, J), IGF-I in normal myometrial cells cultured without any treatment (h, H), IGF-I in normal myometrial cells cultured in the presence of 10–7 M asoprisnil (k, K), TGFβ3 in normal myometrial cells cultured without any treatment (i, I) and TGFβ3 in normal myometrial cells cultured in the presence of 10–7 M asoprisnil (l, L). Bars represent 50 μm. Original magnification 40.
Article Snippet: A mouse monoclonal antibody to human EGF (MAB236, R&D Systems), a goat polyclonal antibody to human IGF-I (AF-291-NA, R&D Systems) and a
Techniques: Expressing, Cell Culture
Journal: Human reproduction (Oxford, England)
Article Title: A novel selective progesterone receptor modulator asoprisnil (J867) down-regulates the expression of EGF, IGF-I, TGFbeta3 and their receptors in cultured uterine leiomyoma cells.
doi: 10.1093/humrep/del035
Figure Lengend Snippet: Figure 4. Effects of asoprisnil on the number of viable leiomyoma and normal myometrial cells cultured for 72 h in the presence of either recombinant epidermal growth factor (EGF), insulin-like growth factor-I (IGF-I) or transforming growth factor β (TGFβ3), as assessed by 3-(4,5-dimethylthi- azol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. Treatment with 100 ng/ml recombinant EGF signifi- cantly increased the number of viable cultured leiomyoma cells compared with untreated control cultures, and the concomitant treatment with 10–
Article Snippet: A mouse monoclonal antibody to human EGF (MAB236, R&D Systems), a goat polyclonal antibody to human IGF-I (AF-291-NA, R&D Systems) and a
Techniques: Cell Culture, Recombinant, MTS Assay, Control